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Cotton Biotechnology: An Efficient Gene Transfer Protocol via Agrobacterium tumefaciens for a Greater Transgenic Recovery

İbrahim İlker ÖZYİĞİT | Yılmaz KAYA

Review | 2022 | Journal of Natural Fibers19 ( 15 )

Due to its economic worth, cotton (Gossypium hirsutum L.) is grown in almost 70 countries and provides income for more than 250 million people. Therefore, producing cotton with having some desired characteristics that includes extended biotic and abiotic stress tolerance, improved fiber quality, promoted nutritional content and increased yield is the main objective for cotton biotechnology. To achieve this goal, many tissue culture and gene transfer techniques are being developed and used throughout the years. As applications for the gene transfer, the Agrobacterium-mediated, particle bombardment and pollen tube pathway-mediated met . . .hods are most successfully used and in conjunction with this, meristematic shoot tips as explants are efficiently utilized in gene transfer methods. In this study, the main objective was to report an efficient protocol for a greater recovery of transgenic cotton plant using Agrobacterium tumefaciens-mediated transformation. For this, one of the cotton strains (Cukurova 1518) cultivated widely in Turkey was chosen and meristematic shoot tips as explant sources, and GFP and NPTII genes as reporter and marker genes were used, respectively. The effective post co-cultivation conditions were provided via using the selection regime in vitro. Finally, the current results showed highly reproducible protocol developed could be used to produce transgenic cotton plants expressing desired traits or can be utilized as a model system to study the expression of particular genes More less

Cotton Agriculture in Turkey and Worldwide Economic Impacts of Turkish Cotton

İbrahim İlker ÖZYİĞİT

Review | 2022 | Journal of Natural Fibers19 ( 15 )

Cotton is a product that provides wealth for humanity with its widespread and compulsory usage, and alongside creating employment opportunities in the producer countries. Due to the availability of suitable land for agriculture, seven countries including Turkey produce close to 80% of cotton worldwide. The average cotton yield in Turkey is above the world average, placing the country close to first place among the major producing countries. On the other hand, Turkey is one of the main countries engaged in organic cotton production by using only non-transgenic seeds and therefore, its cotton as a brand is registered as "GMO Free Turk . . .ish Cotton". This provides a privileged position to the country as "the only country in the world producing GMO-free cotton". Also, the Turkish textile industry is a driving sector for the country in terms of producing high-quality products, bringing foreign exchange to the country and creating a large labor-intensive workforce for the country. In this study, the state of cotton affairs was discussed in detail using relevant data along with its trade and importance in the textile industry in the world and Turkey More less

Economic Impacts of Bt (Bacillus thuringiensis) Cotton

İbrahim İlker ÖZYİĞİT

Article | 2021 | Journal of Natural Fibers19 ( 12 )

In recent years, high-yield production has gained importance due to the decrease in agricultural fields in the world and the rapid increase in the population. Cotton is an important source of income for farmers worldwide and is an important crop, especially for the textile industry. Bt-cotton technology, which entered our lives in 1996, has been quickly adopted in many developed and developing countries. It has been shown to provide advantages in many areas. In particular, the economic growth is gaining importance every year. Additionally, it has also brought an environmentally friendly solution to the crop losses caused by insects . . .and pests over the years, by decreasing chemical spraying. Thus, the cost of farmers was reduced and their health was preserved. It enables farmers to be more profitable by shortening the working hours in the fields and reducing labor force. Moreover, this technology has been shown to increase the yield in cotton production. Fifteen years since the commercialization of Bt-cotton technology was first approved and planted, farmers made profit of million dollars from reduced pesticide use. This study includes detailed information about the contribution of Bt-cotton technology to the leading cotton-producing countrie More less

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